プロトコール
私たちの実験プロトコールをご覧ください。そして、RASTRUM™ を既存の実験ワークフローにいかに簡単に組み込むことができるかをお試しください。
![](/static/27963464d1878ad580c2e76a33d682a5/b2cbe/dffae210-095a-4147-9cd7-804c7f3eb19e_Preview%2BGraphic%2Bwebsite%2B(20).png)
A protocol for the process of handling the RASTRUM™ Removable Models to allow for the implementation of these techniques, such as immunohistochemistry, light-sheet microscopy, advanced tissue-omics and transplantation.
![](/static/79384a94db9b01b144af30c2b0718e44/b2cbe/3b008b8a-9ab8-4de1-857b-171d6d5b3ada_Preview%2BGraphic%2Bwebsite_ELISA%2BAssay.png)
This protocol outlines a method for enzyme-linked immunosorbent assays (ELISA) to assess the proteins secreted by cells in 3D cell models created with RASTRUM™ to provide data on the response from cells in disease state or drug treatment.
![](/static/40be15f9b377cfdf491c4daa0c5f81d3/b2cbe/e40b180e-8f7d-4585-8bce-fdadec3fc480_qPCR%2BGene%2BExpression_Protocol_Transparent.png)
This protocol outlines how qPCR gene expression analysis can be used with RASTRUM™ advanced cell models.
![](/static/7679805f2304ef72c0e7ee9fc3c50e00/b2cbe/602186c2-bd41-4e48-9ec8-0914daec483f_Preview%2BGraphic%2Bwebsite_ProteinExtractionand%2BQuantification.png)
This protocol details how to extract and quantify proteins from RASTRUM™ 3D cell models.
![](/static/88c44ccc25d778885905ca2737e27c28/b2cbe/69ca2dba-1c97-47b0-ab6e-1e38ddd22f45_ILS_Preview%2BIn%2Bsitu%2BViability%2BProtocol.png)
This protocol outlines a method to assess viability of 3D cell models created with RASTRUM™ using fluorescent dyes for distinguishing live and dead cells via fluorescence microscopy.
![](/static/52fd185c8411b6c04b794ec9fc5f97ff/b2cbe/8369250a-f600-417a-8307-a57619efb3ad_RNA%2BExtraction_Protocol_Transparent.png)
A simple step-by-step protocol to extract high quality and purity RNA from a RASTRUM™ advanced cell models for RT-qPCR or RNA sequencing downstream analysis.
![](/static/6abbcf84f1c3f10b37850b5091c8bd15/b2cbe/4455c496-853c-4619-b8ec-53bab30acbee_Incorporation%2Bof%2BDQ%2BCollagen_Protocol_Transparent.png)
This protocol outlines the incorporation of DQ™ Collagen Type I Bovine Skin, Fluorescein Conjugate into RASTRUM™ Matrix.
![](/static/55f756066dd8f74047deae7a614aa0bd/b2cbe/704d5b1a-6fdb-4ce2-87df-a6217901fc6e_AlphaLISA_Protocol_Transparent.png)
A protocol for the Alpha assay using RASTRUM™ 3D cell models.
![](/static/77f7b27af54ded9ed5eb7275c6e802e1/b2cbe/1fad859c-59d1-4173-8700-8b43c90721a4_Preview-Graphic_Protocol_InSitu-Immunofluorescence-Analysis.png)
This protocol outlines a method to analyse protein expression via immunofluorescence in 3D cell models created with RASTRUM™
![](/static/30ee3582f08b0967cecdca0b5f077d58/b2cbe/55065397-24ad-410e-8508-ed49bf5af691_PhenoVue_Protocol.png)
This protocol outlines an easy-to-use method for measuring cell viability, cell proliferation, cytotoxicity and apoptosis using the PhenoVue™ Live/Dead Cell Viability Assay Kit.